Jinma teaches you how to deal with ELISA kit results judgment

EL-C1600N100013-B

WIFI test head can be customized for other specifications

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Kaixin micro test

Interpreting the results of an ELISA kit experiment is one of the most critical steps in the process. Teachers and researchers often pay close attention to this stage, but it's common to face challenges that affect accuracy. To help you better understand and troubleshoot your ELISA results, Shanghai Jinma offers some practical guidance and suggestions:

Common Issues in ELISA Results

1. The color development in all wells, including negative controls, appears too intense.
2. No color develops in any well, even in positive controls.
3. The positive control shows no color, while other samples appear normal.
4. Some samples are not dark enough, making it hard to determine if they're positive or negative.

How to Address These Problems

1. Inconsistent color may be due to insufficient washing, excessive sample loading, or prolonged incubation. Always follow the manufacturerâ€™s instructions carefully. If the issue persists, contact the supplier for further assistance.
2. If no color appears at all, it could be due to missing samples or enzyme solutions, inadequate incubation time, or improper storage of reagents. Double-check all steps and re-run the test. If the problem remains, reach out to the manufacturer for support.
3. A failed positive control might result from incorrect addition or handling. Repeating the test with proper procedures is recommended. If the issue continues, consider requesting a reference standard from the manufacturer.
4. For unclear results, using a microplate reader to measure optical density (OD) values and comparing them against P/N ratios is highly advised for more accurate interpretation.

Shanghai Jinma is committed to providing comprehensive support throughout your ELISA experiments. If you encounter any difficulties, don't hesitate to reach out to us. Our website regularly updates with technical articles and resources to help you stay informed and improve your experimental outcomes. Weâ€™re here to help you achieve reliable and consistent results every time.

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