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Rat Intestinal Fatty Acid Binding Protein (iFABP) ELISA Kit

Basic Information

Abbreviated Chinese Name: Rat Intestinal Fatty Acid Binding Protein (iFABP) ELISA Kit

English Abbreviation: iFABP ELISA Kit | Specification: 48/96T

Chinese Name Alias: Rat Intestinal Fatty Acid Binding Protein (iFABP) Detection Kit

High sensitivity, high efficiency, original imported antibodies, uniform adsorption, excellent performance, high recovery rate, and strong reliability. We offer free testing services and guarantee quality. Our ELISA kits are complete and reliable for all your research needs.

Product Name: Rat Intestinal Fatty Acid Binding Protein (iFABP) ELISA Assay Kit Instruction Manual

English Abbreviation: iFABP ELISA Kit

Specification: 96T / 48T

Shipping Conditions: Transport at 2-8°C using dry ice or ice packs to maintain stability.

Application of the Rat Intestinal Fatty Acid Binding Protein (iFABP) ELISA Kit

For the determination of content or activity in human serum, plasma, tissue, and related liquid samples.

Detection Species: Mouse, Human, Guinea Pig, Rabbit, Pig, Dog, Cattle, Sheep, Chicken, Duck, and other species.

Tip: Please read the instructions carefully before use. This ELISA kit is based on the classical sandwich immunoassay technique and is intended only for research purposes, not for medical diagnosis.

Operation of the Rat Intestinal Fatty Acid Binding Protein (iFABP) ELISA Assay Kit

Description

Instructions for Use of Specimens

1. Samples should be processed as soon as possible after collection, following relevant literature guidelines. Perform the experiment promptly after extraction. If not used immediately, store at -20°C, avoiding repeated freeze-thaw cycles.

2. Avoid using samples containing NaN3, as it may inhibit horseradish peroxidase (HRP) activity.

Washing Method

1. Manual Washing: Carefully remove liquid without touching the plate walls. Place several layers of absorbent paper on a clean surface and gently tap the plate to remove residual liquid. Add at least 0.3 ml of washing buffer into each well, soak for 1–2 minutes, and repeat as needed.

2. Automatic Washing: If an automatic washer is available, use it after becoming familiar with its operation during formal experiments.

Customer Notice for Purchasing the Rat Intestinal Fatty Acid Binding Protein (iFABP) ELISA Assay Kit Instruction Manual

1. Liquid specimens include cell culture supernatant, tissue homogenate, serum, plasma, urine, pleural effusion, ascites, cerebrospinal fluid, etc.

2. Sample Storage: Check samples as soon as possible. Store at 2–8°C for up to one day. For longer storage, freeze at -20°C or -70°C. If collecting samples periodically, pack them quickly and store at -20°C or -70°C.

3. Please inform us in advance about the species, sample size, and specific testing requirements.

Instructions for Using the Rat Intestinal Fatty Acid Binding Protein (iFABP) ELISA Assay Kit

1. When using the kit for the first time, centrifuge all reagent tubes briefly to concentrate the reagents at the bottom of the tube.

2. Leave one well as a blank control for zero adjustment. Do not add any reagents except the final substrate and stop solution. Use this well to set the OD value to zero during measurement.

3. To prevent evaporation, cover the reaction plate with a damp cloth and place it in a closed box during the experiment.

4. Store unused plates or reagents at 2–8°C. Use standard, biotinylated antibody working solution, and HRP-labeled avidin working solution according to the required amount. Do not reuse diluted standards, biotinylated antibody working solutions, or HRP-labeled avidin working solutions.

5. It is recommended to perform duplicate measurements for each sample to ensure accuracy of results.

Purchase our ELISA kits and enjoy free shipping, enhanced after-sales service, and expert technical support. Our team provides professional guidance and uses advanced lab equipment to ensure accurate and reliable experimental data. All our ELISA kits are of high quality, and we offer free returns for any quality issues. You can rely on us for top-notch enzyme-linked immunosorbent assay services!