Overview of Yuanmu Bio ELISA Kits and Their Experimental Guidelines
Yuanmu Bio offers long-term supply of ELISA kits, biological reagents, and other research-grade products. We also provide expert technical support and after-sales service to meet the growing demands of life science research and contribute to scientific progress.
Key Features of the ELISA Kit:
The ELISA (Enzyme-Linked Immunosorbent Assay) is a highly sensitive, specific, and reproducible method used in immunological diagnostics. Its advantages include stable reagents, easy storage, user-friendly operation, and objective result interpretation. Due to these benefits, ELISA has become one of the most widely used techniques in biomedical research and clinical diagnostics.
Important Experimental Rules for ELISA Kits:
1. When pipetting liquid into the wells, ensure that the pipette tip does not touch the liquid inside the well. This prevents droplets from adhering to the tip and ensures accurate dispensing.
2. After all reagents have been added, gently shake the microplate on a flat surface for about 30 seconds to mix the contents thoroughly. Alternatively, you can use the shaking function of a microplate reader if available.
3. When incubating the plate in a water bath, cover it with adhesive film or tape to prevent evaporation and maintain consistent conditions during the reaction.
4. During the washing steps, allow the washing buffer to sit in the wells for at least one minute before aspiration. If a washer is not available, pour out the liquid and gently pat the plate dry using paper towels or newspaper.
5. In case of insufficient washing solution, prepare a phosphate-buffered saline (PBS) solution with pH 7.4 and 0.02 M concentration. Add 0.1% Tween 20 as a detergent. To extend the shelf life, add 1/1000 sodium azide to the solution.
By following these guidelines, researchers can achieve more reliable and consistent results when performing ELISA experiments. Whether you are conducting basic research or diagnostic testing, Yuanmu Bio’s ELISA kits offer high performance and ease of use to support your scientific goals.
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