Understanding the Characteristics and Experimental Guidelines of Yuanmu Bio ELISA Kits
Yuanmu Bio provides long-term supply of ELISA kits, biological reagents, and other scientific research products. We also offer professional technical support and after-sales service to meet the increasing demands of life science research, contributing to the advancement of society.
Key Features of the ELISA Kit:
The ELISA (Enzyme-Linked Immunosorbent Assay) is a highly sensitive, specific, and reproducible method used in immunological testing. It offers stable reagents, easy storage, straightforward operation, and objective results, making it widely applied across various fields of biomedical research and diagnostics.
Guidelines for Conducting ELISA Experiments:
- When dispensing liquid into the wells, ensure that the pipette tip does not touch the liquid inside the well. This helps prevent contamination and ensures accurate droplet delivery by allowing the droplet to naturally fall down the side of the well.
- After all the reagents have been added, gently shake the plate on a flat surface for about 30 seconds to mix the contents thoroughly. Alternatively, use the shaking function of a microplate reader if available.
- When using a water bath for incubation, cover the ELISA plate with adhesive film or tape to minimize evaporation and maintain consistent conditions.
- During washing steps, allow the washing buffer to sit in each well for at least one minute to ensure complete removal of unbound substances. If a washer is not available, carefully blot the plate dry using paper towels or newspaper after pouring out the liquid.
- If the washing solution runs low, you can prepare a phosphate-buffered solution (pH 7.4, 0.02 M) using distilled water and add 0.1% Tween 20 as a washing agent. To extend its shelf life, add 1/1000 sodium azide before storing.
Following these guidelines will help ensure accurate and reliable results when performing ELISA experiments. Proper technique and attention to detail are essential for achieving consistent and meaningful outcomes in your research.
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