What! Does the ELISA kit wash even have patterns? -Huaqiang Electronic Network

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When it comes to experiments, there are always multiple approaches you can take. Whether it's a single item or a complex process, the key is to think creatively and use your brain. One essential step in an ELISA experiment is washing, which plays a critical role in ensuring accurate results. But how exactly is this done? In this article, Shanghai Jinma introduces two common washing methods used in ELISA experiments, helping you understand the best practices for each.

Method 1: Flow Rinse

This method was originally designed for washing bead carriers, using distilled water or even tap water. A special device is attached during the process, allowing the beads to roll continuously under the force of running water. After rinsing for about 2 minutes, the liquid is absorbed, and then the beads are immersed in distilled water for another 2 minutes before being dried.

According to technicians, this approach is more thorough and efficient. It has been proven that the flow rinse method is also effective for washing microtiter plates. To enhance the effect, you can increase the water flow or pressure during the process, so that the water directly impacts the surface of the plate holes, leading to better cleaning results.

Method 2: Soaking

1. First, drain or dry the reaction solution in the wells.

2. Wash the plate once with the washing solution—fill the wells with the solution and then remove it.

3. Soak the plate by filling the wells with the washing solution and letting it sit for 1–2 minutes. Gently shake the plate intermittently. Do not shorten the soaking time arbitrarily.

4. Drain the liquid from the wells. Dry thoroughly using a water pump, vacuum pump, or by blotting the excess liquid onto a clean towel or absorbent paper after removing it.

5. Repeat steps 3 and 4 for 3–4 times (or as specified). In the indirect method, if the background is too high, you may need to increase the number of washes or extend the soaking time.

These are the two main washing techniques commonly used in ELISA experiments. Understanding these methods can significantly improve the quality and reliability of your results. If you have any questions or need further clarification, feel free to leave a comment on our website. You can also contact our sales team directly, and we will arrange for our technical staff to assist you as soon as possible. We're here to help with all your experimental needs!